The 31st Conference on Retroviruses and Opportunistic Infections (CROI), held from March 3 to 6, 2024, is planned as an in-person conference at the Colorado Convention Center in Denver, Colorado. The best and most consequential original research on HIV and related conditions will define the science at the conference. CROI 2024 will also accept important new findings on SARS-CoV-2 and the mpox virus. The in-person conference will also feature innovative interactive sessions, rich poster sessions, and networking opportunities.